Fermentation → Sensory Lab Worksheets designed exactly for KCS TAS 2 + the Causality Bridge Module.

Alfred Gitau Mwaura has written them the way real lab sheets should be: observation → hypothesis


🧪☕ FERMENTATION → SENSORY LAB WORKSHEETS

Reading the Cup as Biochemical Evidence

Institution: Kenya Coffee School
Module: Sensory → Fermentation → Roast → Cup
Level: TAS 2 (Sensory Intelligence)


🧫 LAB 1 — ACID TYPE IDENTIFICATION

Which acids were designed during fermentation?

Objective

To identify dominant organic acids in the cup and trace them to fermentation pathways.

Cup Sample ID: ___________

A. Sensory Detection (Taste First)

Tick all that apply:

  • ☐ Juicy / citrus
  • ☐ Crisp / apple-like
  • ☐ Sparkling / cola-like
  • ☐ Creamy / yogurt-like
  • ☐ Sharp / vinegary
  • ☐ Harsh / drying

B. Acid Mapping

Sensory CueLikely AcidDetected (✓)
Citrus, juicyCitric acid
Green appleMalic acid
SparklingPhosphoric acid
CreamyLactic acid
Sharp sourAcetic acid
Harsh bitternessQuinic acid

C. Fermentation Hypothesis

Dominant fermentation type (circle one):

  • Washed (controlled)
  • Extended washed
  • Lactic / inoculated
  • Anaerobic
  • Uncontrolled / over-fermented

Reasoning (chemical, not opinion):



🍬 LAB 2 — SWEETNESS ORIGIN ANALYSIS

Is sweetness built or missing?

Objective

To determine whether perceived sweetness originates from sugar preservation, fermentation efficiency, or roast exposure.

A. Sweetness Perception

Rate on a scale of 1–5:
Sweetness intensity: 1 ☐ 2 ☐ 3 ☐ 4 ☐ 5 ☐

Sweetness character (tick):

  • ☐ Honey-like
  • ☐ Caramel
  • ☐ Candy-like
  • ☐ Thin / fleeting
  • ☐ Absent

B. Chemical Interpretation

ObservationLikely Cause
Deep sweetnessHigh sucrose + controlled fermentation
Thin sweetnessSugar loss during fermentation
No sweetnessPoor cherry maturity or degradation

Likely fermentation outcome:



C. Roast Interaction Check

Does roast appear to:

  • ☐ Preserve sweetness
  • ☐ Expose lack of sugars
  • ☐ Degrade sugars

Trainer note: Roast reveals what fermentation built.


🧈 LAB 3 — BODY & MOUTHFEEL TRACE

Texture as biochemical evidence

Objective

To link mouthfeel to lipid + polysaccharide preservation during fermentation and processing.

A. Mouthfeel Description

Tick dominant sensation:

  • ☐ Thin
  • ☐ Round
  • ☐ Creamy
  • ☐ Oily
  • ☐ Dry

B. Compound Mapping

MouthfeelChemical Group
CreamyLipids + polysaccharides
ThinLow structural compounds
OilyExcess surface lipids
DryPhenolic dominance

C. Fermentation Link

Likely fermentation impact on body:

  • ☐ Preserved structure
  • ☐ Degraded polysaccharides
  • ☐ No significant effect

Explain:



🌬️ LAB 4 — AROMATIC IDENTITY & MICROBIAL SIGNALS

What microbes spoke through aroma?

Objective

To identify aromatic families and link them to microbial activity.

A. Aroma Detection (Dry & Wet)

Tick all perceived:

  • ☐ Fruity
  • ☐ Floral
  • ☐ Winey
  • ☐ Nutty
  • ☐ Solvent-like
  • ☐ Funky

B. Aroma → Microbial Link

Aroma TypeLikely Source
Fruity / floralEsters (yeast activity)
WineyEthanol → acetic pathway
FunkyUncontrolled microbes
Clean & clearStable fermentation ecology

C. Quality Judgment (Circle one)

Aroma expression is:
Clean / Expressive / Overripe / Defective

Chemical justification:



⚠️ LAB 5 — DEFECT FORENSICS

Diagnosing fermentation failure

A. Primary Defect Detected

  • ☐ Sharp sour
  • ☐ Medicinal
  • ☐ Musty
  • ☐ Fermented / rotten
  • ☐ Astringent

B. Causality Chain

StageObservation
Sensory symptom
Chemical imbalance
Fermentation failure
Roast roleExposure / Amplification

C. Final Verdict (Complete the sentence)

“This defect is caused by __________________ during fermentation,
and the roast __________________ the issue.”


🧠 LAB 6 — FULL CUP BACK-TRACING (CAPSTONE)

Blind Cup Code: ___________

  1. Top 3 sensory signals detected:
  2. Dominant chemical groups:
  3. Most likely fermentation style:
  4. Roast interaction:
    • Preserved / Exposed / Destroyed
  5. Overall cup quality (chemical reasoning):

🎯 ASSESSMENT CRITERIA (Trainer Use)

Students are evaluated on:

  • Chemical reasoning (not guessing)
  • Logical fermentation linkage
  • Clear sensory language
  • Ability to separate cause vs amplifier

Correct reasoning beats correct answers.


KCS Teaching Line (Use Often)

The tongue detects.
The brain diagnoses.
The process explains.


Alfred Gitau Mwaura says “Cupping is forensic science” ☕🧬